FIELD: biotechnology.
SUBSTANCE: invention is the truncated mutant luciferase MLM4 of Metridia longa with the size of -16 kDa with improved properties for use as a genetically encoded bioluminescent reporter for visualisation of molecular processes in living cells. The amino acid substitutions I69L, N74E, K125V, W139F are introduced in the sequence of the truncated luciferase MLM4 of Metridia longa with the size of -16 kDa.
EFFECT: invention enables to provide the luciferase with improved thermal stability and the spectrum shift to long-wave region, which determines an overall increase in sensitivity of the bioluminescent reporter when used in living cells and organisms.
4 dwg, 1 tbl, 3 ex
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