FIELD: chemistry.
SUBSTANCE: group of inventions relates to biotechnology, gene and protein engineering and specifically to recombinant plasmid DNA pG1-Rm7, which facilitates synthesis of hybrid protein G1-Rm7 in Escherichia coli cells, which is capable of biding the tumour necrosis factor and has bioluminescence of luciferase Renilla muelleri, where said plasmid DNA includes the nucleotide sequence SEQ ID NO: 1 and can be in medicine. The invention also relates to the protein pG1-Rm7 having molecular weight of 65.4 kDa, consisting of a single-strand anti tumour necrosis factor antibody, a GGSGGS peptide and modified luciferase Renalla muelleri and characterised by SEQ ID NO: 2.
EFFECT: invention enables to obtain a highly sensitive reporter for detecting a tumour necrosis factor via bioluminescent analysis.
2 cl, 4 dwg, 3 ex
| Title |
Year |
Author |
Number |
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2014 |
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|
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2021 |
- Bashmakova Evgeniia Evgenevna
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|
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2022 |
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|
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2012 |
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|
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2020 |
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|
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2014 |
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2013 |
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2019 |
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|
RU2728652C1 |
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2012 |
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2012 |
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