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2 495 930

(13)

(51)

IPC

C12N9/14(2006-01-01)

C12R1/01(2006-01-01)

C12R1/465(2006-01-01)

(21) (22)

Application

2012127024/10, 2012-06-27

(24)

Start date

2012-06-27

(22)

Actual filing date

2012-06-27

(45)

Published

2013-10-20

(72)

Inventor

Sharova Natal'Ja Jur'Evna

(73)

Holder

Gosudarstvennoe Nauchnoe Uchrezhdenie Vserossijskij Nauchno-Issledovatel'Skij Institut Pishchevykh Aromatizatorov, Kislot I Krasitelej Rossijskoj Akademii Sel'Skokhozjajstvennykh Nauk Vniipakk Rossel'Khozakademii)

METHOD OF PRODUCTION OF GLYCOSIDASE INHIBITOR Russian patent published in 2013 - IPC C12N9/14 C12R1/01 C12R1/465

Abstract RU 2495930 C1

FIELD: biotechnology.

SUBSTANCE: fermentation of nutritional medium is carried out based on corn starch hydrolysates by actinomycete. Then ultrafiltration of the obtained filtrate of the culture liquid is carried out with an activity of 4250±250 IU/cm³ through membranes with cut-off of 5 kDa. The filtrate obtained is concentrated under vacuum of 0.082 mPa at a temperature of 25-30°C. It is clarified at the temperature of 30-40°C. Its ultrafiltration is carried out again through the membrane with cut-off of 1 kDa. The obtained glycosidase inhibitor is dried and ground.

EFFECT: invention enables to obtain the glycosidase inhibitor with increased activity.

1 tbl, 4 ex

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RU 2 495 930 C1

Authors

Sharova Natal'Ja Jur'Evna

Dates

2013-10-20—Published

2012-06-27—Filed