METHOD FOR OBTAINING NANOSIZED DELIVERY SYSTEM OF FRAGMENTS OF NUCLEIC ACIDS AND THEIR ANALOGUES TO CELLS OF MAMMALS Russian patent published in 2013 - IPC C12N15/00 B82B3/00 

Abstract RU 2499045 C1

FIELD: biotechnologies.

SUBSTANCE: invention proposes a production method of a nanosized delivery system of fragments of nucleic acids (FNA) and their analogues to cells of mammals. A suspension of TiO2 nanoparticles with concentration of 1-2 mg/ml in 0.1-0.5 M solution of NaCl is obtained. With that, TiO2 particles have the size of 3-20 nm, and mainly 3-5 nm, and are contained in amorphous or crystalline anatase or brookite form. The obtained TiO2 suspension is mixed with water solution of polylysin with concentration of 20 mg/ml in the ratio of TiO2 to polylysin, which is equal to 1:(0.05-0.8). The mixture is incubated at room temperature during at least 30 minutes. Then, to the obtained suspension of polylysin-containing nanoparticles there added is 5-70 mcL of FNA solution with concentration of 10-4-10-7 M and incubated in 0.1-0.5 M solution of NaCl at room temperature during 20-30 minutes. Nanocomposite TiO2-PL FNA with capacity as per FNA of 0.2-60 nmol/mg is obtained.

EFFECT: invention allows simplifying a production method of FNA delivery system and shortening its duration.

4 cl, 3 dwg, 14 ex

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RU 2 499 045 C1

Authors

Zarytova Valentina Filippovna

Levina Asja Saulovna

Repkova Marina Nikolaevna

Ismagilov Zinfer Rishatovich

Shikina Nadezhda Vasil'Evna

Dates

2013-11-20Published

2012-03-05Filed