METHOD OF BIOTESTING OF WATER CONTAMINATED WITH CRUDE OIL ACCORDING TO LENGTH OF TEST PLANT ROOTS Russian patent published in 2013 - IPC G01N33/18 

FIELD: ecology.

SUBSTANCE: test plant is chosen, uniform seeds of test plant placement is carried out on the filter paper in the control and test Petri dish of 10 cm in diameter. Then 5.0 ml of water is poured in each control and the test Petri dish with 4-8 replicates of watering, at that the level of liquid in the dishes must be below the surface of the seeds. At that in the control Petri dish the water sample with a zero concentration of crude oil is taken, and in the test dishes before watering the water emulsions of test crude oil with different concentrations are prepared. Then the seeds of test plant in Petri dishes are watered with prepared water emulsions with the specified concentration of crude oil for 72 hours, and the concentration of crude oil in different water emulsions is increased until the moment of measurement of root length when the germination of round red radish is not less than 50 %.

EFFECT: assessment of dangerous levels of contamination of water objects with crude oil, by means of improving the accuracy of indices of effects of crude oil of different volume concentration in the aqueous solution on the root growth of plants in a particular area.

3 cl, 12 dwg, 3 tbl, 1 ex