FIELD: biotechnologies.
SUBSTANCE: method for inhibition of RNA-polymerase ferment activity is proposed. Inhibition is performed by introduction to a transcriptional system of an inhibitor based on at least one compound containing organic macrocyclic complex with encapsulated ion of transition metal, which has been chosen from the group including 1,8-bis(2-fluorobora)-2,7,9,14,15,20-hexaoxa-3,6,10,13,16,19-hexaasa-4,5,11,12-tetraphenyl-17,18-diamino-bicyclo[6.6.6]eicosa-3,5,10,12,16,18-hexaen(2-) ferrum(2+); 1,8-bis(2-fluorobora)-2,7,9,14,15,20-hexaoxa-3,6,10,13,16,19-hexaasa-4,5,11,12-tetraphenyl-17,18-dimethyl-bicyclo[6.6.6]eicosa-3,5,10,12,16,18-hexaen(2-) cobalt(2+); 1,8-bis(2-phenylbora)-2,7,9,14,15,20-hexaoxa-3,6,10,13,16,19-hexaasa-4,5,11,12,17,18-hexa(methyl thio)-bicyclo[6.6.6]eicosa-3,5,10,12,16,18-hexaen(2-) ruthenium(2+); 1,8-bis(2-fluorobora)-2,7,9,14,15,20-hexaoxa-3,6,10,13,16,19-hexaasa-4,5,11,12-tetraphenyl-17,18-cyclohexanedimercaptylbicyclo[6.6.6]eicosea-3,5,10,12,16,18-hexaen(2-) ferrum(2+); 1,12-bis-(tert-butylbora)-2,11,13,22,23,32-hexaoxa-3,10,14,21,24,31-hexasapentacyclo[11,11.11.04.9015,20025.30]ditri-akonta-3,9,14,20,24,30-hexaen(2-) ferrum(2+).
EFFECT: method provides for inhibition of activity of RNA-polymerase at micromolar concentration of inhibitor and enlarges the range of inhibitors of RNA-polymerase.
22 ex
| Title | Year | Author | Number |
|---|---|---|---|
| FUSED PROTEIN OF THIOREDOXIN AND INFESTIN 4 DOMAIN, METHOD FOR PREPARING IT, EXPRESSION PLASMID DNA CODING FUSED PROTEIN, AND BACTERIUM Escherichia coli TRANSFORMED BY THIS PLASMID DNA | 2012 |
|
RU2528251C2 |
| METHOD OF USING BINARY SYSTEM CONSISTING OF AGENTS OF VARIOUS NATURES TO DEVELOP NEW CLASS OF ANTIBACTERIAL MEDICINAL PRODUCTS | 2021 |
|
RU2810089C2 |
| RNA-FRAGMENT OF POTATO X-VIRUS INTENDED FOR FOREIGN GENE TRANSLATION | 1991 |
|
RU2005788C1 |
| METHOD FOR SCREENING OF ANTITUMOR PREPARATIONS - PARP1 INHIBITORS BASED ON BIOCHEMICAL METHODS OF ANALYSIS | 2015 |
|
RU2639535C2 |
| SAFE CELL REPORTER SYSTEM FOR SEARCHING FOR NUCLEOSIDE AND NON-NUCLEOSIDE INHIBITORS OF RNA-DEPENDENT RNA-POLYMERASE SARS-CoV-2 | 2024 |
|
RU2836343C1 |
| RECOMBINANT DNA AS AGENT FOR INHIBITION OF RNA FUNCTION IN LIVING ORGANISM | 1990 |
|
RU2136751C1 |
| METHOD OF IN VITRO REPRODUCTION OF PROTEOLYTIC ACTIVITY OF HEPATITIS C VIRUS PROTEASE NS3 | 1995 |
|
RU2149185C1 |
| METHOD OF OBTAINING RIBONUCLEIC ACID | 2009 |
|
RU2439156C2 |
| TEST SYSTEM FOR HUMAN PROTEIN KINASE GSK3β INHIBITOR SCREENING | 2012 |
|
RU2528059C2 |
| RECOMBINANT PLASMID DNA pQe30_PS-CFP2/TurboYFP_MBP7, CODING PROTEIN PS-CFP2/TurboYFP_MBP7, STRAIN OF Escherichia coli BL21(DE3)/pQe30_PS-CFP2/TurboYFP_MBP7 - SAID PROTEIN PRODUCENT AND METHOD OF OBTAINING PROTEIN PS-CFP2/TurboYFP_MBP7 | 2009 |
|
RU2430161C2 |
