FIELD: medicine.
SUBSTANCE: invention relates to medicine and process for producing heterogeneous preparation of various dispersities based on bromelain and chitosan, including the immobilization of the enzyme preparation in a buffer solution, incubation and washing. Immobilization of bromelain is carried out on an acid-soluble chitosan matrix of medium molecular or high molecular weight; as a buffer solution for immobilization, a tris-glycine buffer with pH 8.5–9.0 for medium molecular weight and pH 8.5 for high molecular weight chitosan is used. Incubation is carried out at room temperature for 4 hours for medium molecular weight and 5 hours for high molecular weight chitosan; the precipitate formed is washed with tris-HCl buffer.
EFFECT: invention provides reduction in consumption of the wound healing agent due to the high stability of the preparation at temperatures higher than physiological and prolonged action of the preparation.
1 cl, 3 dwg, 2 ex
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