SYNTHETIC ANTIGEN HAVING ABILITY TO FIX AUTOANTIBODIES TO MUSCARINE M2-RECEPTOR Russian patent published in 2013 - IPC C07K14/705 A61K38/17 G01N33/53 A61P9/06 

FIELD: biotechnologies.

SUBSTANCE: invention describes creation of an artificial conformational antigen including peptide fragments consisting of two extracellular loops of muscarine M2-receptor, which are cross-linked with disulphide bond, and which is able to fix autoantibodies to this receptor with sensitivity exceeding the sensitivity for a linear prototype or mechanical mixture of data of linear peptide fragments. Synthetic antigen represents an individual chemical compound containing peptide corresponding to the sequence of the 1-st extracellular loop and peptide of the 2-nd extracellular loop, which are linked with disulphide connection between Cys⁹⁶ and Cys¹⁷⁶ residues (structure of antigen is disclosed in the formula and description). As per the invention, there described are methods for obtaining and using an antigen for creation of a diagnostics set for reveal of antibodies to muscarine M2-receptor in blood of patients with idiopathic arrhythmia using an enzyme-linked immunosorbent assay (ELISA) method.

EFFECT: improvement of the method.

5 cl, 1 dwg, 3 tbl, 4 ex