FIELD: medicine.
SUBSTANCE: invention discloses a method of solid-phase immune-enzyme assay wherein as an antigen, an equimolar mixture of synthetic peptides is immobilised on plastic with the mixture containing: nonapeptide (position 125-133), tridecapeptide (position 208-218) of an amino acid sequence of a human β1 adrenoreceptor molecule and a chimeric construct of the same peptides jointed by a disulphide bond. The presented method shows substantially greater sensitivity in detecting the presence of β1-adrenoreceptor autoimmune antibodies in blood plasma and serum in the patients with dilatation cardiomyopathy.
EFFECT: invention is also applicable for diagnosing the patients with various cardiovascular diseases.
1 dwg, 4 ex
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Authors
Dates
2012-06-10—Published
2011-04-15—Filed