FIELD: medicine.
SUBSTANCE: invention discloses a method of solid-phase immune-enzyme assay wherein as an antigen, an equimolar mixture of synthetic peptides is immobilised on plastic with the mixture containing: nonapeptide (position 125-133), tridecapeptide (position 208-218) of an amino acid sequence of a human β1 adrenoreceptor molecule and a chimeric construct of the same peptides jointed by a disulphide bond. The presented method shows substantially greater sensitivity in detecting the presence of β1-adrenoreceptor autoimmune antibodies in blood plasma and serum in the patients with dilatation cardiomyopathy.
EFFECT: invention is also applicable for diagnosing the patients with various cardiovascular diseases.
1 dwg, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| SYNTHETIC ANTIGEN ABILITY TO BIND β-ADRENORECEPTOR AUTOANTIBODIES | 2007 |
|
RU2356576C1 |
| SYNTHETIC ANTIGEN HAVING ABILITY TO FIX AUTOANTIBODIES TO MUSCARINE M2-RECEPTOR | 2012 |
|
RU2502743C1 |
| SYNTHETIC OLIGOYPEPTIDES SPECIFICALLY INTERACTING WITH ANTIBODIES RAISED TO HEPATITIS C VIRUS, METHODS OF DIAGNOSIS HCV-INFECTION (VARIANTS) | 1994 |
|
RU2104284C1 |
| POLYCLONAL ANTIBODY SPECIFICALLY REACTING WITH PRIONIC PROTEIN IN CEREBRAL TISSUE OF MAMMALIANS AND DIAGNOSTIC REAGENT UPON ITS BASIS | 2005 |
|
RU2281511C1 |
| IX PADUA FACTOR ANTIBODIES | 2017 |
|
RU2770006C2 |
| IMMUNOGENIC COMPOSITIONS BASED ON SYNTHETIC PEPTIDES MIMICKING ACTUAL DETERMINANTS OF HIV1 gp120 | 2014 |
|
RU2577132C1 |
| ANAPHYLATOXIN C5A ANTIBODIES TO HUMAN | 2019 |
|
RU2731516C1 |
| METHODS OF TREATING CONDITIONS ASSOCIATED WITH MASP-2 DEPENDENT COMPLEMENT ACTIVATION | 2012 |
|
RU2743409C2 |
| PEPTIDE COMPOSITION POSSESSING IMMUNOREGULATORY PROPERTY | 2004 |
|
RU2266297C1 |
| METHOD OF DETERMINING HIGH LEVEL OF AUTOANTIBODIES TO HUMAN PLASMINOGEN AND DEGRADATION PRODUCTS THEREOF AND DIAGNOSTIC TEST SYSTEM THEREFOR | 2014 |
|
RU2597783C2 |
