FIELD: medicine.
SUBSTANCE: invention provides a method for producing a heterogenous skin cell population implying the sampling of a skin biopsy material at a depth of 2 mm, tissue homogenisation in 0.9% aqueous sodium chloride at temperature +23 … +25°C, recovery of homogenate, filtration of the homogenate through an inert filter cloth with the pore diameter of 20 mcm, centrifugation of the homogenate at 400 g for 5 minutes at temperature +23 … +25 C.
EFFECT: skin cell separation and viability preservation.
2 cl, 1 dwg
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Authors
Dates
2013-12-27—Published
2012-07-25—Filed