FIELD: bioengineering.
SUBSTANCE: plants or plant material containing VLP localised in apoplast are produced. Mixture of protoplasts/spheroplasts and apoplasts is produced by the plant or plant material treatment by the multicomponent enzymatic mixture destructing the cell walls, and containing cellulase. Fraction of protoplast/spheroplast and fraction of apoplast are produced from mixture of protoplasts/spheroplasts and apoplasts. Fraction of apoplast is extracted, wherein it contains VLP. VLP is extracted from apoplast fraction.
EFFECT: invention ensures with high efficiency the extraction of the virus-like particle.
43 cl, 12 dwg, 15 tbl, 9 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PRODUCING PLANT PROTEIN | 2010 |
|
RU2567012C2 |
| PRODUCTION OF VIRUS-LIKE PARTICLES IN PLANTS | 2011 |
|
RU2655431C2 |
| HEMAGGLUTININ-CONTAINING CHIMERIC VIRUS-LIKE PARTICLES, SIMILAR TO PARTICLES OF FLU VIRUS | 2010 |
|
RU2569195C9 |
| INCREASING VARIABLE-FREE PARTICLES OUTPUT IN PLANTS | 2012 |
|
RU2682752C2 |
| OBTAINING VIRUS-LIKE PARTICLES OF INFLUENZA VIRUS IN PLANTS | 2014 |
|
RU2742607C1 |
| OBTAINING VIRUS-LIKE PARTICLES OF INFLUENZA VIRUS IN PLANTS | 2014 |
|
RU2705555C2 |
| HEMAGLUTININ MUTANTS OF THE INFLUENZA VIRUS | 2019 |
|
RU2800938C2 |
| RECOMBINANT VACCINE AGAINST AVIAN FLU AND APPLICATION THEREOF | 2009 |
|
RU2559527C2 |
| OBTAINING PICORNAVIRUS-LIKE PARTICLES IN PLANTS | 2013 |
|
RU2693431C2 |
| RECOMBINANT VIRUS-LIKE PARTICLES (VLP) USING BOVINE IMMUNODEFICIENCY VIRUS GROUP-SPECIFIC ANTIGEN (GAG) PROTEIN | 2016 |
|
RU2734118C2 |
