METHOD FOR EARLY DIAGNOSIS OF SECONDARY IMMUNODEFICIENCY IN CHILDREN UNDER CONDITIONS OF EXCESSIVE ALUMINIUM CONTAMINATION OF BIOLOGICAL MEDIA Russian patent published in 2025 - IPC G01N33/68 G01N33/49 G01N33/58 C12Q1/6806 C12Q1/6827 C12Q1/686 C12Q1/6883 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to preventive medicine, human ecology and paediatrics, and can be used for early diagnosis of secondary immunodeficiency in children under conditions of excessive aluminium contamination of biological media. Aluminium content is determined in the child's urine. Blood is analysed for level of specific immunoglobulin G (IgG) to aluminium and level of T-regulatory lymphocytes CD127^-. Polymerase chain reaction is used to determine polymorphism of the FAS cell death receptor gene and the TLR4 toll-like receptor gene. If the content of aluminium in the child's urine is 0.012 mg/dm³ and more, if the level of IgG to aluminium is not less than 1.2 times higher than norm of 0.0-0.1 standard units, with a decrease of not less than 1.1 times the level of CD127^- compared to the lower limit of the norm equal to 1.2%, as well as in the presence of the CC genotype of the FAS C14405T (rs1159120) gene and the GG genotype of the TLR4 A8595G (rs1927911) gene, the child is diagnosed with developing secondary immunodeficiency in conditions of excessive aluminium contamination of biological media.

EFFECT: method provides early diagnosis of secondary immunodeficiency in children with excessive aluminium contamination of biological media by determining toxicological, laboratory and genetic indicators of the child’s health.

2 cl, 2 tbl, 2 ex