FIELD: chemistry.
SUBSTANCE: invention relates to molecular biology and genetics. Disclosed is a method for qualitative and quantitative analysis of lipids that are strongly bound with genomic DNA, which comprises steps of isolating genomic DNA bound with lipids from cells using a detergent method, hydrolysis of DNA with a hydrolysing enzyme, separating lipids from the chloroform-methanol-water mixture at 37°C, evaporating the solvent, stabilising lipids with 2,6-di-tert-butyl-p-cresol, mixing the lipids with TMSH, chromatography with a gas chromatograph, followed by mass spectrometry.
EFFECT: method can be used to decode the lipid code of genomic DNA, ie, to determine the area of localisation of lipids that are strongly bound with genomic DNA for research and therapeutic purposes.
5 dwg
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