FIELD: biotechnology.
SUBSTANCE: monitoring the carriage of a modified vaccine strain (nOPV2) type 2 of the poliomyelitis virus in healthy individuals, diagnosing individuals with suspected poliomyelitis and patients with poliomyelitis. Diagnosis is carried out using RNA isolated from biological samples and wastewater for the purpose of diagnosis and/or epidemiological monitoring, as well as for solving research problems to study the properties of the modified poliovirus vaccine type 2 (nOPV2).
EFFECT: method for detecting nOPV2 RNA by real-time RT-PCR, which consists of original oligonucleotide primers and an oligonucleotide labeled with a fluorescent label (fluorescent probe).
1 cl, 3 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DETECTING JUNIN VIRUS RNA BY REAL-TIME RT-PCR | 2023 |
|
RU2822164C1 |
| METHOD OF DETECTING NIPAH VIRUS USING REAL-TIME RT-PCR | 2022 |
|
RU2816270C2 |
| METHOD FOR DETECTING HENDRA HENIPAVIRUS RNA BY REAL-TIME RT-PCR | 2023 |
|
RU2822161C1 |
| METHOD FOR DETECTING MEASLES VIRUS BY REAL-TIME RT-PCR | 2023 |
|
RU2822430C1 |
| SET FOR DETECTING SARS-COV VIRUS BY REAL-TIME RT-PCR METHOD | 2020 |
|
RU2744198C1 |
| METHOD OF DETECTING MONKEY POX VIRUS OF MONKEYPOX TYPE BY REAL TIME PCR (MPX AMP PS) | 2023 |
|
RU2803898C1 |
| METHOD FOR TICK-BORNE ENCEPHALITIS VIRUS DETECTION BY REAL-TIME RT-PCR METHOD | 2019 |
|
RU2744187C1 |
| METHOD FOR DETECTING RNA OF SCHMALLENBERG DISEASE VIRUS IN FARM ANIMALS | 2018 |
|
RU2696306C1 |
| METHOD FOR DETECTING THE DNA OF THE HEPATITIS B VIRUS IN BIOLOGICAL MATERIAL WITH A LOW VIRAL LOAD BASED ON TWO-STAGE PCR WITH DETECTION BY THREE TARGETS IN REAL TIME | 2020 |
|
RU2763173C1 |
| METHOD FOR DETECTING GENOME OF PARAINFLUENZA VIRUS OF TYPE 3 IN BOVINE ANIMALS | 2018 |
|
RU2696069C2 |
