FIELD: biotechnology; medicine.
SUBSTANCE: invention refers to biotechnology, particularly to medical diagnostics. Disclosed is a kit of reagents, comprising oligonucleotide primers and fluorescent-labeled DNA probes, which enables reliable detection of specific genomic areas of microorganisms of the genus Brucella with simultaneous identification of epidemiologically significant B.abortus, B.suis, B.canis, B.melitensis species in samples of different origin by real-time polymerase chain reaction.
EFFECT: invention can be used in medical and health care institutions to support diagnosis of a specific pathology (brucellosis) when examining material from a patient (suspected of disease) and indicating pathogenic biological agents (PBA) in environmental objects; as an accelerated preliminary test when performing a biological method of investigating and identifying suspicious cultures; for epidemiological monitoring.
1 cl, 4 dwg, 5 tbl, 3 ex
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