METHOD FOR DETERMINING INDICATIONS FOR RADIATION THERAPY IN PATIENTS SUFFERING TUMOURS BY PREDICTION OF EFFECTIVENESS Russian patent published in 2014 - IPC G01N33/49 G01N33/52 A61P35/00 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to oncology and radiology, and can find application in treating the patients suffering cerebral malignancies. The method for determining the indications for a radiation therapy in the patients by prediction of the effectiveness including blood sampling, gamma-irradiation of a portion of this sample in vitro, incubation of the irradiated and non-irradiated portions of the blood samples, staining of the DNA components of both blood portions with a DNA-specific fluorescent dye, leukocyte count in the irradiated portion of the blood sample, leukocyte count in the non-irradiated blood sample, staining of all DNA containing blood components, determination of DI_i that is the DNA amount in all the DNA containing blood components at one leukocyte of the irradiated portion of the sample and DI_n that is the DNA amount in all the DNA containing blood components at one leukocyte of the irradiated portion of the blood sample, calculation of DI_n/DI_i. Blood is sampled additionally, added with an aqueous solution containing ferrous iron ions in the concentration of 50-75 mg/l in the amount of 8-14% of the blood sample amount. The additional blood sample is then incubated for 15-30 minutes that is followed by gamma irradiation of a portion of the additional blood sample. Then the irradiated and non-irradiated portions of the additional blood sample are incubated for 2.5-3.5 hours with leukocyte counting in the irradiated and non-irradiated portions of the additional blood sample, staining of the all DNA containing components of the portions of the additional blood sample and determination of DI_{i add} that is the DNA amount in all the DNA containing components of the additional blood sample at one leukocyte of the irradiated portion of the blood sample and DI_{n add} that is the DNA amount in all the DNA containing components of the additional blood sample at one leukocyte of the non-irradiated portion of the blood sample. Then, the relation DI_{n add}/DI_{i add} is calculated, and if observing DI_{n add}/DI_{i add}> DI_n/DI_i being 20-35% and DI_n/DI_i>1, the radiation therapy is considered to be indicated.

EFFECT: invention provides higher effectiveness of the method used for determining the indications for the radiation therapy in the patients suffering glioblastomas.

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