FIELD: medicine.
SUBSTANCE: invention refers to medicine, namely to oncology and radiology, and can find application in treating the patients suffering cerebral malignancies. The method for determining the indications for a radiation therapy in the patients by prediction of the effectiveness including blood sampling, gamma-irradiation of a portion of this sample in vitro, incubation of the irradiated and non-irradiated portions of the blood samples, staining of the DNA components of both blood portions with a DNA-specific fluorescent dye, leukocyte count in the irradiated portion of the blood sample, leukocyte count in the non-irradiated blood sample, staining of all DNA containing blood components, determination of DIi that is the DNA amount in all the DNA containing blood components at one leukocyte of the irradiated portion of the sample and DIn that is the DNA amount in all the DNA containing blood components at one leukocyte of the irradiated portion of the blood sample, calculation of DIn/DIi. Blood is sampled additionally, added with an aqueous solution containing ferrous iron ions in the concentration of 50-75 mg/l in the amount of 8-14% of the blood sample amount. The additional blood sample is then incubated for 15-30 minutes that is followed by gamma irradiation of a portion of the additional blood sample. Then the irradiated and non-irradiated portions of the additional blood sample are incubated for 2.5-3.5 hours with leukocyte counting in the irradiated and non-irradiated portions of the additional blood sample, staining of the all DNA containing components of the portions of the additional blood sample and determination of DIi add that is the DNA amount in all the DNA containing components of the additional blood sample at one leukocyte of the irradiated portion of the blood sample and DIn add that is the DNA amount in all the DNA containing components of the additional blood sample at one leukocyte of the non-irradiated portion of the blood sample. Then, the relation DIn add/DIi add is calculated, and if observing DIn add/DIi add> DIn/DIi being 20-35% and DIn/DIi>1, the radiation therapy is considered to be indicated.
EFFECT: invention provides higher effectiveness of the method used for determining the indications for the radiation therapy in the patients suffering glioblastomas.
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Authors
Dates
2014-02-10—Published
2012-12-12—Filed