DIAGNOSTIC TECHNIQUE FOR PACTREATIC INFECTIOUS NECROSIS VIRUS IN SALMON BY POLYMERASE CHAIN REACTION Russian patent published in 2014 - IPC G01N33/50 

Abstract RU 2508547 C2

FIELD: medicine.

SUBSTANCE: there are performed two rounds of a polymerase chain reaction with the primers B37 - B853r and B123 - B320r to detect a fragment of a segment B coding a RNA-dependent RNA-polymerase of a pancreatic infectious necrosis virus that is followed by an electrophoretic measurement of a size of an amplified fragment of a nucleotide sequence. If the electrophoregram shows the fragments of the length of 860 and/or 240 pn in the analysed sample, the pancreatic infectious necrosis virus is diagnosed in salmon.

EFFECT: invention is applicable for the diagnostic purposes in scientific research institutions, veterinary laboratories, and fish farms.

2 dwg, 3 tbl, 3 ex

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RU 2 508 547 C2

Authors

Kandrina Natalija Jur'Evna

Lomakina Natal'Ja Fedorovna

Zav'Jalova Elena Aleksandrovna

Guljukin Mikhail Ivanovich

Dates

2014-02-27Published

2012-03-05Filed