FIELD: biotechnology.
SUBSTANCE: invention relates to genetic engineering and biotechnology and can be used to produce a chimeric human angiogenin protein. Recombinant plasmid is proposed, pPICZaA:ZZ_ANG, which contains a synthetic gene for the chimeric protein of human angiogenin (SEQ ID NO: 1), consisting of the amino acid sequence of two IgG-binding domains of the protein A (SEQ ID NO: 2) and human angiogenin (SEQ ID NO: 3), linked together by a peptide linker (SEQ ID NO: 4). Plasmid has a size of 4228 bp. Strain of yeast is proposed, Pichia pastoris X-33/pPICZaA:ZZ-ANG, which is the producer of the recombinant chimeric protein of human angiogenin ZZ-ANG and obtained by transforming yeast by Pichia pastoris X-33 linearized plasmid pPICZaA:ZZ-ANG.
EFFECT: group of inventions provides an active recombinant chimeric human angiogenin secreted into the culture medium in high yield.
2 cl, 4 dwg, 5 ex
| Title |
Year |
Author |
Number |
| ARTIFICIAL GENE, CODING CIMERIC PROTEIN OF HUMAN ANGIOGENIN, CHIMERIC PLASMID pJZZ-A, ENSURING EXPRESSION OF GENE OF CHIMERIC PROTEIN OF HUMAN ANGIOGENIN IN Escherichia coli AND STRAIN OF Escherichia coli BL21(DE3)/pJZZ-A-PRODUCENT OF RECOMBINANT CHIMERIC PROTEIN OF HUMAN ANGIOGENIN |
2012 |
- Ramazanov Jurij Akhmetovich
- Beklemishev Anatolij Borisovich
|
RU2512527C2 |
| RECOMBINANT CHIMERIC PROTEIN SUMO3-apoA-I FOR PRODUCING MATURE HUMAN APOLIPOPROTEIN A-I, Pichia pastoris YEAST STRAIN - PRODUCER OF RECOMBINANT CHIMERIC PROTEIN SUMO3-apoA-I AND METHOD OF PRODUCING MATURE HUMAN APOLIPOPROTEIN A-I |
2015 |
- Mamaev Aleksej Lvovich
- Beklemishev Anatolij Borisovich
|
RU2605326C1 |
| RECOMBINANT PLASMID DNA ENCODING CHIMERIC INTERFERON ALPHA2b, RECOMBINANT STRAIN OF YEAST P. PASTORIS X33 - PRODUCER OF CHIMERIC INTERFERON ALPHA2b, AND METHOD FOR PRODUCING THIS PROTEIN |
2020 |
- Beklemishev Anatolij Borisovich
- Pykhtina Mariya Borisovna
|
RU2764787C1 |
| CHIMERA PLASMID PZZSA FOR EXPRESSION OF CHIMERA PROTEIN ANGIOGEN AND STRAIN OF ESCHERICHIA COLI BL21(DE3) PZZSA AS PRODUCER OF RECOMBINANT CHIMERA HUMAN PROTEIN ANGIOGEN |
2002 |
- Mertvetsov N.P.
- Ramazanov Ju.A.
- Majstrenko V.F.
|
RU2221043C2 |
| STRAIN YEAST PICHIA PASTORIS PS107(pPIC9HAbIL-2) AS PRODUCER OF HYBRID PROTEIN CONSISTING OF HUMAN PLASMA BLOOD ALBUMIN AND HUMAN INTERLEUKIN-2, RECOMBINANT PLASMID pPIC9HAbIL-2 AND METHOD FOR ITS CONSTRUCTING |
2006 |
- Padkina Marina Vladimirovna
- Zinov'Eva Julija Grigor'Evna
- Sambuk Elena Viktorovna
- Smirnov Mikhail Nikolaevich
|
RU2315105C1 |
| RECOMBINANT PLASMID FOR PHOSPHOLIPASE GENE EXPRESSION IN PICHIA PASTORIS YEAST, PICHIA PASTORIS YEAST STRAIN-PHOSPHOLIPASE PRODUCER |
2009 |
- Borshchevskaja Larisa Nikolaevna
- Gordeeva Tat'Jana Leonidovna
- Bavykina Natalija Borisovna
- Sineokij Sergej Pavlovich
|
RU2409671C1 |
| RECOMBINANT PLASMID FOR PHYTASE GENE EXPRESSION IN PICHIA PASTORIS YEAST (VERSIONS), PICHIA PASTORIS YEAST STRAIN -PHYTASE PRODUCER (VERSIONS) |
2009 |
- Borshchevskaja Larisa Nikolaevna
- Gordeeva Tat'Jana Leonidovna
- Bavykina Natalija Borisovna
- Sineokij Sergej Pavlovich
|
RU2409670C1 |
| PICHIA PASTORIS X3351 RECOMBINANT YEAST STRAIN - BOVINE LEUKEMIA VIRUS GP 51 VACCINE PROTEIN PRODUCER |
2017 |
- Usoltsev Konstantin Valerevich
- Skvortsov Evgenij Vladimirovich
- Khammadov Nail Ildarovich
|
RU2701692C2 |
| METHYLOTROPHIC YEAST STRAIN PICHIA PASTORIS YST-HSA-PDI1, PRODUCING RECOMBINANT HUMAN SERUM ALBUMIN |
2019 |
- Bobik Tatiana Vladimirovna
- Mokrushina Iuliana Anatolevna
- Smirnov Ivan Vitalevich
- Gabibov Aleksandr Gabibovich
|
RU2733423C1 |
| RECOMBINANT PLASMID, METHOD FOR ITS CONSTRUCTION AND YEAST STRAIN KOMAGATAELLA PASTORIS - PRODUCER OF IMMUNE INTERFERON GAMMA OF A DOG |
2020 |
- Muzaev Dmitrij Mikhajlovich
- Padkina Marina Vladimirovna
|
RU2756852C2 |
