FIELD: biotechnology.
SUBSTANCE: method of producing an antibody or a fragment thereof, a method of preventing disulphide bond recovery during purification of an antibody or a fragment thereof, a method of purifying an antibody or a fragment thereof from a liquid mixture, as well as a method for reducing the amount of reducing agents during purification of the antibody or fragment thereof. Said methods involve administering a mixture comprising an antibody or a fragment thereof, host cell fragments and impurities to a filtration step through activated carbon, and then chromatography on protein A.
EFFECT: invention provides purification of proteins in their native conformation with constant degree of purity in various batches.
15 cl, 7 dwg, 3 ex
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