FIELD: chemistry.
SUBSTANCE: invention relates to biotechnology. The method involves performing multiple substitutions on hydrophilic residues identified by analysis of a database of selected, stable scFv sequences. Also described is a single-chain antibody obtained using said method and a composition containing same. The invention can be used in medicine.
EFFECT: method for analysis of sequences and rational strategies to improve solubility of immunobinders and particularly single-chain antibodies (scFv).
13 cl, 7 dwg, 14 tbl, 4 ex
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