FIELD: chemistry.
SUBSTANCE: present invention relates to biotechnology. Disclosed is a method of improving functional properties of an antibody or antigen-binding fragment thereof, for which the heavy chain belongs to the human VH1b family, which comprises performing mutagenesis in frameworks with replacement of the amino acid in position 78 of the heavy chain in the AHo numbering system (position 67 in the Kabat numbering system) into leucine or isoleucine.
EFFECT: method improves the stability and solubility of antibodies and antigen-binding fragments thereof.
2 cl, 17 dwg, 33 tbl, 10 ex
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