FIELD: chemistry.
SUBSTANCE: invention relates to medical microbiology and a method of determining activation of plasminogen with bacteria. The method involves adding protamine sulphate to a prepared supernatant fluid, incubating the obtained mixture, depositing cells by centrifuging, incubating the supernatant fluid with the protamine sulphate, depositing protein and detecting activation of plasminogen with bacteria from the amount of split arginine, content of which is determined by Sakaguchi method from the red colour of the sample.
EFFECT: invention enables to detect activation of plasminogen with bacteria in vitro using protamine.
4 tbl, 4 ex
| Title |
Year |
Author |
Number |
| PLASMINOGEN UROKINASE TYPE MODIFIED ACTIVATOR, DNA SEQUENCE, RECOMBINANT PLASMID, STRAIN-PRODUCER, METHOD FOR PREPARING PLASMINOGEN UROKINASE TYPE MODIFIED ACTIVATOR AND PHARMACEUTICAL COMPOSITION ELICITING THROMBOLYTIC EFFECT |
2001 |
- Gurskij Ja.G.
- Belogurov A.A.
- Bibilashvili R.Sh.
- Grigor'Eva N.V.
- Del'Ver E.P.
- Minashkin M.M.
|
RU2247777C2 |
| RECOMBINANT POLYPEPTIDE WITH HUMAN PLASMINOGEN PROPERTIES TO CONVERT INTO PLASMIN IF ACTIVATED WHICH CATALYSES FIBRIN SPLITTING, DNA FRAGMENT CODING POLYPEPTIDE, RECOMBINANT PLASMID DNA FOR POLYPEPTIDE EXPRESSION AND TRANSFORMED Escherichia coli CELL - POLYPEPTIDE PRODUCER |
2009 |
- Bibilashvili Robert Shalvovich
- Belogurov Anatolij Aleksandrovich
- Gurskij Jaroslav Georgievich
- Minashkin Mikhail Mikhajlovich
- Skamrov Andrej Viktorovich
- Skrypina Natalija Alekseevna
- Feoktistova Evgenija Sergeevna
|
RU2432396C2 |
| STRAIN SAROCLADIUM KILIENSE - A PRODUCER OF LONGOLITINE - A COMPLEX OF FIBRINOLYTIC AND THROMBOLYTIC ENZYMES AND A METHOD FOR PRODUCING LONGOLITINE |
2019 |
- Khokhlov Nikolaj Valerevich
- Velikanov Evgenij Viktorovich
- Brovkin Aleksej Nikolaevich
|
RU2716984C1 |
| METHOD FOR DETERMINING TISSULAR PLASMINOGEN ADJUVANT |
2003 |
- Ajsina R.B.
- Bulygin O.Ju.
- Varfolomeev S.D.
- Vojushina T.L.
- Mukhametova L.I.
- Mamaev A.N.
- Momot A.P.
|
RU2252421C1 |
| METHOD OF DETERMINING PLASMINOGEN AND PLASMIN INHIBITORS LEVELS IN BLOOD PLASMA |
1998 |
- Stepanov V.M.
- Vojushina T.L.
- Teren'Teva E.Ju.
- Mil'Gotina E.I.
- Nevedrova O.E.
- Makarov V.A.
- Kogan A.E.
|
RU2121690C1 |
| TWO-COMPONENT PHARMACEUTICAL COMPOSITION HAVING FIBRINOLYTIC EFFECT |
2020 |
- Bibilashvili Robert Shalvovich
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| METHOD OF DETERMINING THE CONTENT OF MUC1 PROTEOLISE PRODUCTS AND DIAGNOSTIC TEST SYSTEM FOR ITS IMPLEMENTATION |
2016 |
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RU2676258C2 |
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2009 |
- Bibilashvili Robert Shalvovich
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| METHOD FOR DEFIBROTIDE BIOLOGICAL ACTIVITY DETERMINATION, BASED ON EUGLOBULIN APPLICATION |
2012 |
- Inoni Terentsio
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RU2627177C2 |
| RECOMBINANT PLASMID DNA pBK415 CODING POLYPEPTIDE OF RECOMBINANT TISSULAR ACTIVATOR OF PLASMINOGEN OF HUMAN BEING, CELL LINE Cricetulus griseus CHO 1F8-PRODUCER OF RECOMBINANT TISSULAR ACTIVATOR OF PLASMINOGEN OF HUMAN BEING, AND METHOD FOR OBTAINING AND SEPARATION OF POLYPEPTIDE HAVING ACTIVITY OF TISSULAR ACTIVATOR OF PLASMINOGEN |
2012 |
- Grigor'Eva Ol'Ga Vasil'Evna
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RU2500817C1 |
