METHOD FOR DEFIBROTIDE BIOLOGICAL ACTIVITY DETERMINATION, BASED ON EUGLOBULIN APPLICATION Russian patent published in 2017 - IPC C12Q1/37 C12Q1/56 C12Q1/68 G01N33/48 

FIELD: biotechnology.

SUBSTANCE: defibrotide, mammal euglobulin and a substrate specific for the plasmin which, by reaction with the plasmin, gives a measurable product, are brought into contact. The substrate specific for plasmin is a compound of formula A₁-A₂-A₃-X, where A₁ and A₂ are non-polar amino acids, A₃ is lysine or arginine, and X is the measurable product. The measurable product is selected from the group consisting of para-nitroaniline and 2-naphthylamine. The amount of obtained product is measured at successive time points to determine defibrotide biological activity. The invention provides accurate and precise measurement of defibrotide activity even in the presence of impurities such as, for example, RNA, heparin, degraded defibrotide.

EFFECT: sensitive method to detect defibrotide concentrations, and provide good correlation up to the maximum concentration values.

14 cl, 3 dwg, 4 tbl