FIELD: medicine.
SUBSTANCE: method concerns quantitative picamilon measurement. Solutions of a measured substance (concentration of 0.00002 g/ml) and a reference sample are prepared. A solvent for preparing the measured solutions is presented by 0.1 M sodium hydroxide. The reference sample is methyl orange. An optical density of the solution of the measured substance and the reference sample is measured in a spectrophotometer at an analytical wave length of 261 nm. The results are calculated by formula with recalculation factor 0.7505 introduced therein.
EFFECT: method allows for increasing reproducibility of the measurement results, reducing cost price, labour-intensiveness and analysis error, and unifying the analysis procedure.
4 ex
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Authors
Dates
2014-05-27—Published
2013-01-29—Filed