FIELD: biotechnology, molecular biology. SUBSTANCE: method is carried out by culturing the strain of Chinese hamster ovarian cells (CHO) transformed with plasmid that contains human erythropoietin gene in growth and accumulative nutrient media. Accumulative medium has the following components (in 1 l): Fetaclone 11 (10 ml), glutamine 200 mg, HEPES 3.57 g and a mixture of media RPM1, 1640 and DMEM taken at equal parts. Strain is obtained by transformation of Chinese hamster ovarian cell (CHO) к 1DHFR with plasmid pBD carrying the complete human erythropoietin gene followed by selection on medium containing 1 mM methotrexate. Strain is deposited at number VSKK (P)-651 D. EFFECT: high yield of erythropoietin under industrial conditions. 5 cl, 6 dwg, 1 tbl, 7 ex
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