FIELD: biotechnology.
SUBSTANCE: nucleotide sequences are formed, encoding the hybrid proteins EPO-TR 1.6, EPO-TR 4 and EPO-TR 6. Protein EPO-TR 1.6 is recombinant human erythropoietin fused with a fragment TR 1.6 of the human protein MUC1. Protein EPO-TR 4 is recombinant human erythropoietin fused with a fragment TR 4 of the human protein MUC1. The hybrid protein EPO-TR 6 is recombinant human erythropoietin fused with a fragment TR 6 of the human protein MUC1. Hybrid proteins are produced by the roller cultivation in the suitable conditions the modified mammalian cell line CHO containing a nucleotide sequence encoding the protein with subsequent isolation of the hybrid protein from the culture fluid.
EFFECT: invention enables to produce the hybrid recombinant human erythropoietin having the prolonged action.
4 cl, 4 dwg, 7 tbl, 9 ex
| Title | Year | Author | Number |
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| PLASMID FOR EXPRESSION OF HUMAN RECOMBINANT BLOOD CLOTTING FACTOR IX, CHO CELL PRODUCING HUMAN RECOMBINANT BLOOD CLOTTING FACTOR IX AND METHOD OF PRODUCING SAID FACTOR | 2014 |
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| STRAIN OF CELLS OF CHINESE HAMSTER OVARIES - PRODUCER OF RECOMBINANT ANTIBODY AGAINST TUMOUR NECROSIS FACTOR ALPHA OF HUMAN BEING | 2014 |
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| RECOMBINANT PLASMID DNA OF pBiPr-ABIgA2m1F16-ht TO OBTAIN RECOMBINANT IMMUNOGLOBULIN A OF ISOTYPE IgA2m1 | 2016 |
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| RECOMBINED PLASMID DNA CODING CHIMERIC ANTIBODY AGAINST HUMAN TUMOUR NECROSIS FACTOR-ALPHA, EUKARYOTIC CELL LINE PRODUCING CHIMERIC ANTIBODY, AND METHOD OF CHIMERIC ANTIBODY OBTAINMENT | 2013 |
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| RECOMBINANT PLASMID DNA PBIPR-ABIGA1FI6-HT FOR OBTAINING RECOMBINANT IMMUNOGLOBULIN A IGOTH IGA1 | 2016 |
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