FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to microbiology, namely to using bacterial beta-lactamase for diagnosing in vitro and visualising, diagnosing and treating in vivo. A method for real-time detecting pathogenic bacteria in an individual consists in the fact that a fluorogenic substrate sample for bacterial beta-lactamase is administered into the individual or taken from the individual; visualising the individual or the sample for the presence of the fluorescent product of beta-lactamase activity on the substrate; generating signals at a wave length emitted by the fluorescent beta-lactamase product, and real-time detecting pathogenic bacteria in the individual. The fluorogenic substrate represents CNIR5, CNIR5-QSY22, CNIR7, CNIR7-TAT, CNIR9 or CNIR10. A method for monitoring the developing pathophysiological condition related to pathogenic bacteria in the individual. A method for screening the compounds possessing a therapeutic effect on pathogenic Mycobacterium in the individual. A method for visualising pathogenic bacteria with the fluorogenic substrate for bacterial beta-lactamase.
EFFECT: using the declared invention enables providing better visualisation of pathogenic bacteria and monitoring of efficacy of the therapeutic compounds.
20 cl, 26 dwg, 1 tbl, 14 ex
