FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to the chemical-pharmaceutical industry, particularly to a method for quantitation of anthracene derivatives in horse sorrel roots. The raw material is extracted in 70% ethanol; an aliquote of the prepared extract is diluted in an alkaline-ammonia solution; an optical density of the solution is measured at a wave length of 520 nm; the total anthracene derivates are re-counted at 8-O-β-D-glucoside emodin that is used as a reference substance.
EFFECT: method enables reducing labour input, reducing time and stages of the process, and decreasing the analyte loss.
1 dwg, 1 ex
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