METHOD FOR MEASURING ANTHOCYANES IN CRUDE DRUGS Russian patent published in 2015 - IPC G01N33/15 

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to chemical-pharmaceutical industry, and can be used in drug quality control centres and analytical laboratories when measuring anthocyanes in such crude drugs, as bilberry, black chokeberry, black currant, etc. The presented method for measuring anthocyanes involves recovering anthocyanes from crude drugs in alcohol water mixtures containing 0.5-1.0% muriatic acid with the ethanol concentration of less than 70% and measuring anthocyanes quantitatively by optical density in 95% ethanol containing 0.5% ammonium as compared to a reference solution at analytical wavelength of peak absorption within the range of 611-630 nm with using a reference solution of cyanidine-3-O-glucoside or with using its specific absorption in 95% ethanol containing 0.5% ammonium.

EFFECT: higher sensitivity and result reproducibility, as well as shortening the analysis procedure as compared to its prototype.

3 dwg, 7 ex