FIELD: medicine.
SUBSTANCE: wings of ilium are punctured in an anterior and posterior one-third of the wings with two trocars being inserted into each wing. The bone marrow (BM) is collected by simple aspiration, aspiration irrigation or a combination thereof at an underpressure of 0.6 Atm with using a device. The bone marrow preparation device comprises a disposable multi-channel closed system, an aspiration collection unit and a perfusion unit. The group of inventions also refers to a method for assessing the prepared bone marrow. The effect is ensured by automatic control of myeloaspiration by preparing a biological material with using a special designed device for the bone marrow collection.
EFFECT: using the given method for preparing the bone marrow provides preparing the sterile bone marrow rich in viable multipotent mesenchymal stromal and hemopoietic progenitor cells.
7 cl, 1 ex, 1 dwg, 1 tbl
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Authors
Dates
2014-07-20—Published
2012-11-29—Filed