FIELD: biotechnology and medicine.
SUBSTANCE: method of detecting the chimeric DNAJB1-PRKACA transcript in clinical tissue samples from patients with fibrolamellar liver carcinoma using real-time polymerase chain reaction is described, the said method is characterized by the use of specific oligonucleotide sequences, primers, with the following SEQ ID NO: 1, NO. 2, the following SEQ ID NO: 4, NO. 5, supplemented with specially adapted fluorescent probes of the following SEQ ID NO: 3, ID NO: 6.
EFFECT: invention makes it possible to differentiate fibrolamellar carcinomas from other types of liver tumors in order to select the optimal treatment tactics for a specific disease, and also improves diagnostic accuracy through the use of original oligonucleotide sequences and optimized reaction conditions.
1 cl, 2 dwg, 7 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR QUANTITATIVE DETERMINATION OF MUTATIONS F317L AND F359V/C OF THE KINASE DOMAIN BCR-ABL IN PATIENTS WITH CHRONIC MYELOID LEUKEMIA RESISTANT TO THERAPY WITH TYROSINE KINASE INHIBITORS | 2019 |
|
RU2698461C1 |
| METHOD FOR QUANTITATIVE DETERMINATION OF G250E MUTATION OF BCR-ABL KINASE DOMAIN FOR PATIENTS WITH CHRONIC MYELOID LEUKOSIS RESISTANT TO THYROSINKINASE INHIBITORS THERAPY | 2016 |
|
RU2620076C1 |
| METHOD FOR DETECTING CHIMERIC EWSR1 GENE TRANSCRIPTS ASSOCIATED WITH EWING'S SARCOMA | 2021 |
|
RU2759810C1 |
| ANALYSIS METHOD OF EML4-ALK TRANSLOCATIONS ASSOCIATED WITH SENSITIVITY OF LUNG CANCER TO ANTITUMOUR TARGET THERAPY | 2012 |
|
RU2509153C1 |
| METHOD FOR DIAGNOSING ACUTE LEUKEMIA AND KITS FOR ITS IMPLEMENTATION | 2021 |
|
RU2806591C2 |
| METHOD FOR IDENTIFYING CHROMOSOMAL TRANSLOCATIONS LEADING TO THE DEVELOPMENT OF MALIGNANT BLOOD DISEASES (LEUKOSES), DUE TO APPLYING OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) | 2004 |
|
RU2286798C2 |
| DIAGNOSING TECHNIQUE FOR NON-SMALL-CELL LUNG CANCER AND SET FOR IMPLEMENTING THEREOF | 2010 |
|
RU2445627C1 |
| DETERMINING mRNA LEVEL REDUCTION IN GENES ITGA9, HYAL1 AND HYAL2 AS METHOD OF DIAGNOSING NON-SMALL CELL LUNG CANCER AND SET FOR ITS REALISATION | 2008 |
|
RU2403575C2 |
| DETERMINATION OF GENE ZG16 mRNK LEVEL REDUCTION AS METHOD OF LARGE INTESTING CANCER DIAGNOSTICS AND SET OF ITS REALISATION | 2009 |
|
RU2395234C1 |
| METHOD OF PROGNOSTIC ASSESSMENT OF DEVELOPMENT OF HEPATOCELLULAR CARCINOMA BASED ON DETERMINATION OF POLYMORPHISM OF HUMAN IFNAR-1 GENE AND A SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS | 2022 |
|
RU2811763C1 |
