SYSTEM OF OLIGONUCLEOTIDE PRIMERS AND PROBE FOR DETECTING DNA MYCOPLASMA BOVIS Russian patent published in 2021 - IPC C12Q1/68 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology. Disclosed is a system of primers and a probe, which enables specific detection of Mycoplasma bovis DNA by PCR in real time in DNA preparation extracted from biological material from cattle or raw material of animal origin for biological industry and products obtained from it. System includes the following components: a pair of oligonucleotide primers, complementary to a species-specific region in the Mycoplasma bovis sugar kinase gene and having forward and reverse primer activity and having a structure M.bov1 F 5'-GAACTAAGATTGGCGTCAAGACA-3', M.bov1 R 5'-GTTGCTGCAAGCGACACTAA-3', and a fluorescent labeled hydrolysis probe for identifying Mycoplasma bovis DNA during a real-time PCR reaction, complementary to a species-specific portion of the Mycoplasma bovis sugar kinase gene, located in sequence flanked by said primers and having structure M.bov1 Pr 5'-FAM CAGGTCATTATAGTCAATAGATTCAGTA BHQ1-3'.

EFFECT: disclosed system of oligonucleotide primers and probes enables determining the presence of Mycoplasma bovis DNA in the analyzed material and differentiating Mycoplasma bovis from phylogenetically related Mycoplasma bovigenitalium mycoplasmas.

1 cl, 2 dwg, 2 ex