FIELD: biotechnology.
SUBSTANCE: invention relates to the clinical laboratory diagnosis of virus infections, and may be used for genotyping herpes virus 6 (HHV-6). The method enables to determine one-nucleotide polymorphisms specific for HHV-6A and HHV-6B in the U67 HHV-6 gene via real-time PCR using primers HHV6F 5'-CGGATACAGTAAGACGGGATAT-3' and HHV6R 5'-ACGTAAGCTTGCACAATGC-3' and two fluorescently labeled probes HHV6AZ F1-GCAATAGATTTGAGAACGCGCGGCAT-Q1 and HHV6BZ F2-GCAATAGATTCGGAAATGCGGCAT-Q2, where the presence of HHV-6A or HHV-6B is indicated when the exponential accumulation of the fluorescence signal is registered in the respective probes channels. The invention may be used for genotyping herpes virus 6 (HHV-6).
EFFECT: rapid and reliable identification of viruses HHV-6A and HHV-6B.
2 dwg, 1 ex
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