FIELD: instrumentation.
SUBSTANCE: invention relates to microscopy. According to the method, microobject image formation is implemented with the help of a confocal scanning microscope. In the process of radiation focusing onto the survey plane and in the process of radiation focusing on the reception slot diaphragm one ensures change of the diffraction maximum sizes of each point image within the focusing plane, narrowing it in one direction relative to the other ones. One performs additional scanning of the microobject being surveyed in several different directions, simultaneously recording the coordinates of movement of the microobject being surveyed and photoelectric signals. Orientation of the direction of narrowing of the diffraction maximum and the slot diaphragms remains unchanged relative to the scanning direction. One performs consolidated electronic processing of photoelectric signals registered in the primary and the additional directions of scanning and the coordinates of movement of the microobject being surveyed that correspond to such directions.
EFFECT: improved detalisation (increased resolution) of the image of the microobject under survey.
20 cl, 4 dwg
Title | Year | Author | Number |
---|---|---|---|
METHOD OF MICRO OBJECTS INVESTIGATION AND NEAR-FIELD OPTICAL MICROSCOPE FOR ITS IMPLEMENTATION | 2016 |
|
RU2643677C1 |
LASER DEVICE TO EXAMINE FIELD OF MICROOBJECTS BY BEAM ACTION (VERSIONS) | 2002 |
|
RU2199729C1 |
CONFOCAL IMAGE SPECTRUM ANALYSER | 2014 |
|
RU2579640C1 |
METHOD OF CONFOCAL SCANNING THREE-DIMENSIONAL MICROSCOPY AND CONFOCAL SCANNING TOMOGRAPHIC MICROSCOPE | 1999 |
|
RU2140661C1 |
METHOD FOR STUDYING PHASED OBJECTS AND DEVICE | 0 |
|
SU1696974A1 |
MULTICHANNEL CONFOCAL MICROSCOPE (VERSIONS) | 2014 |
|
RU2574863C1 |
DEVICE FOR CONTINUOUS CONTROL OF PARAMETERS OF HEXAHEDRAL FIBER-OPTIC ROD DURING ITS DRAWING | 1992 |
|
RU2020410C1 |
METHOD MEASURING VELOCITY OF DIFFUSING MICROOBJECTS | 2002 |
|
RU2223504C1 |
METHOD OF AUTHENTICATING AND MANUFACTURING QUALITY OF PROTECTIVE HOLOGRAMS MADE BASED ON DIFFRACTION MICROSTRUCTURES, AND DEVICE FOR IMPLEMENTATION THEREOF | 2019 |
|
RU2722335C1 |
ANGLE METER | 1994 |
|
RU2116618C1 |
Authors
Dates
2014-08-10—Published
2012-06-25—Filed