METHOD FOR DETERMINING OXIDATIVE PROTEIN MODIFICATION IN SUBSTANCE POOL OF AVERAGE MOLECULAR WEIGHT IN BLOOD SERUM, PLASMA, ERYTHROCYTE, AND IN URINE Russian patent published in 2014 - IPC G01N33/86 G01N21/27 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, particularly to clinical biochemistry, and aims at determining oxidative protein modification in a substance pool of an average molecular weight in a biological medium accompanying any pathological conditions by biochemical examination. The biological medium specified in the blood plasma, erythrocyte or urine is sampled; proteins are deposited by adding 10% trichloroacetic acid; if observing sedimentation, a centrifugation cycle follows at 1000 rpm for 15 minutes; thereafter, 2,4-dinitrophenylhydrazine 0.05 M in hydrochloric acid 2 M is added; the sample is centrifuged at 1000 rpm for 20 minutes; and if observing sedimentation, the sediment is washed twice in ethanol-ethylacetate (1:1), dried on a water bath for 10 minutes and dissolved in urea 8 M; the sample is kept in a boiling water bath for 10 minutes until dissolved completely; the prepared solution is analysed by spectrophotometry. The method is applicable both for single use, and for monitoring of the postoperative oxidation protein modification and average molecule levels.

EFFECT: method provides higher information value of biochemical tests, reducing consumption of the biological material.

9 tbl, 2 ex