FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to medicine and biotechnology. What is presented is a method for improving the immunosuppressive properties of mesenchymal stromal cells of fatty tissues involving human mesenchymal stromal cell culture in DMEM/F12 medium containing penicillin 100 unit/ml and streptomycin 100 unit/ml, glutamine 2 mM, sodium pyruvate 1 mM, 10% foetal bovine serum in the presence of anti-inflammatory TNF-α factor in the concentration of 20 ng/ml and IFN-γ factor in the concentration of 30 ng/ml characterised by the fact that the culture process is carried out in the environment consisting of CO2 5% and air 95% at temperature 37°C for 48 hours.
EFFECT: invention enables improving the immunosuppressive activity of mesenchymal stromal cells and can be used in transplantology and pharmacology.
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