GENETIC CONSTRUCT CONTAINING hGDNF CONTROLLED BY TEMPERATURE-SENSITIVE PROMOTOR FOR REGULATED NEUROTROPHIC FACTOR EXPRESSION IN MAMMALIAN CELLS AND BODIES DIRECTLY Russian patent published in 2014 - IPC C12N15/00 

FIELD: medicine.

SUBSTANCE: invention refers to molecular biology and medicine. What is presented is a genetic construct on a basis of the vector plasmid pEGFP-N1 with a neomycin resistance gene, containing human glial cell-derived neurotrophic factor (GDNF) gene with heat shock elements (HSE) 4-8 and green fluorescent protein (GFP) gene controlled by a thermally regulated promoter of heat shock protein hsp70 gene of Drosophila melanogaster. The invention can be used in therapy of neurodegenerative disorders, traumatic adromia, as well as in cerebral ischemic stroke in mammals (including in humans), as a decrease of temperature activation threshold (39 to 42 degrees Celsius) of GDNF therapeutic gene expression enables reducing a negative effect of high temperatures on human body when using the construct containing the GDNF therapeutic gene for treating the neurodegenerative disorders that is ensured by using hsp 70 of Drosophila melanogaster.

EFFECT: temperature regulated temporary activation of GDNF is ensured by stimulating neural differentiation of neural precursors, as well as prevents the negative consequences of transgenic factor hyperexpression.

26 dwg, 7 ex