FIELD: chemistry; biochemistry.
SUBSTANCE: invention relates to biotechnology, specifically to obtaining cell population and can be used in cell transplantology and tissue engineering in order to obtain cell material for restoring nerve tissue damaged due to injury, stroke or neurodegenerative diseases. The method involves isolating a population of stromal cells from fat tissue, immunosorting based on using antibodies against the brain-derived neurotropic factor (BDNF) receptor (TrkB) to obtain an essentially homogeneous sub-population of TrkB-expressing cells for their culturing in a standard medium for mammal cells and differentiation induction through transfer into a medium which contains a neural differentiation inducing substance - BDNF combined with 5-azacytidine or retinoic acid combined with 5-azacytidine.
EFFECT: invention enables to obtain a population of neural differentiation induced stromal cells of fat tissue.
7 cl, 5 dwg, 4 tbl, 6 ex
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