NOOTROPIC COMPOSITION BASED ON POLYPEPTIDE COMPLEXES RECOVERED FROM NEURONAL PROGENITOR CELLS UNDER HYPOXIC CONDITIONS, AND A METHOD FOR PRODUCTION THEREOF Russian patent published in 2020 - IPC C12N5/95 A61K31/00 

FIELD: biochemistry.

SUBSTANCE: invention relates to biochemistry, in particular to a method for preparing a nootropic composition based on polypeptide complexes recovered from neuronal progenitor cells under hypoxic conditions, as well as to a nootropic composition based on polypeptide complexes recovered from neuronal progenitor cells under hypoxic conditions. Said composition is characterized by that it contains proteins and polypeptides with molecular weight from 5 kDa to 250 kDa, cerebral neurotrophic factor (BDNF) of at least 130 pg/ml and a glial neurotrophic factor (GDNF) of at least 30 pg/ml, vascular endothelium growth factor (VEGF) of not less than 90 pg/ml, total protein weight of not less than 1 mg/ml of the preparation. Cell culture of neuronal progenitor cells from which the composition is produced demonstrates neuronal expression β-tubulin III, molecules of adhesion of nerve cells (PSA-NCAM) and consists of 95±5 % TUBB3+ cells.

EFFECT: invention enables to efficiently obtain a protein-peptide composition, which includes not only small peptides, but also full-length protein neuronal growth and differentiation factors, the effectiveness of which is certainly higher than that of short peptides, and immunogenicity is absent.

2 cl, 4 ex, 2 dwg