EXPRESSION VECTOR FOR TRANSGENIC INTRODUCTION INTO CELLS AND TISSUES OF MAMMALS WITH REGULATED NON-VIRAL PROMOTOR Russian patent published in 2013 - IPC C12N15/64 C12N15/85 

FIELD: biotechnologies.

SUBSTANCE: proposed vector is designed on the basis of a vector plasmid pEGFP-Nl, containing a DNA fragment, which codes a promotor of a heat shock protein gene hsp70 Drosophila melanogaster and a regular sequence upstream, containing heat shock elements (HSE) in different quantities, a polylinker zone, a gene of green fluorescent protein (GFP) and a gene of stability to neomycin, at the same time the promotor is capable of activation under action of temperature of heat shock of mammals or under toxic effect. Such vector is activated as temperature increases to 38°C or in case of toxic impact at transgenic cells or tissue of mammals. Activity of a promotor within the vector may be regulated, i.e. it is possible to cause either its hyperactivity or its weak leak, or to block activity of this promotor by saturation of the regulatory area with HSE elements.

EFFECT: invention may be used to produce transgenic preparations, where an investigated or used gene will be under control of a regulated non-viral promotor.

3 cl, 20 dwg, 2 ex