NOOTROPIC COMPOSITION BASED ON POLYPEPTIDE COMPLEXES ISOLATED FROM NEURONAL PROGENITOR CELLS UNDER HEAT SHOCK AND METHOD FOR PRODUCTION THEREOF Russian patent published in 2021 - IPC C12N5/95 A61K31/00 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology, particularly to a method for producing a nootropic composition based on polypeptide complexes isolated from neuronal progenitor cells under heat shock. Said composition is characterised by the fact that it contains proteins and polypeptides with a molecular weight from 3 kDa to 250 kDa, 60% whereof have a molecular weight in the range from 10 to 50 kDa, brain-derived neurotrophic factor (BDNF) no less than 120 pg/ml, and glial cell line-derived neurotrophic factor (GDNF) no less than 40 pg/ml, vascular endothelial growth factor (VEGF) not less than 60 pg/ml, total protein weight no less than 1 mg/ml of the preparation. The cell culture of neuronal progenitor cells therein, wherefrom the composition is produced, demonstrates the expression of a β3-tubulin neuronal marker, a nerve cell adhesion molecule (PSA-NCAM), and consists of 99.5±2.45% TUBB3⁺ cells.

EFFECT: invention allows effectively producing a protein-peptide composition including not only small peptides, but also full-size protein neuronal growth and differentiation factors the effectiveness whereof is invariably higher than in short peptides and immunogenicity is not present.

2 cl, 2 dwg, 4 ex