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2 658 605

(13)

(51)

IPC

C12N9/26(2006-01-01)

C12N9/24(2006-01-01)

(21) (22)

Application

2016126682, 2016-07-01

(24)

Start date

2016-07-01

(22)

Actual filing date

2016-07-01

(45)

Published

2018-06-21

(72)

Inventor

Chajka Oleg VasilevichElikhanov Adlan MajrbekovichGlazova Natalya VladimirovnaDudnik Olga GennadevnaMelnikova Yana Viktorovna

(73)

Holder

Glazova Natalya Vladimirovna

METHOD FOR PRODUCING HYALURONIDASE FROM TESTIS OF LARGE CATTLE Russian patent published in 2018 - IPC C12N9/26 C12N9/24

Abstract RU 2658605 C2

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology. Method for obtaining hyaluronidase from testes of large cattle is proposed. Method comprises extracting the ground raw material with 0.1 N acetic acid solution for 4 hours. Extract is subsequently subjected to centrifugation/separation, ion exchange sorption on macroporous sulfocationite, followed by elution of the enzyme from the carrier with ammonia eluent of pH 10.0–11.0 at an elution rate of 80–120 ml/cm²h.

EFFECT: invention provides an increase in yield and an improvement in the quality of the target product.

6 cl, 4 ex

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RU 2 658 605 C2

Authors

Chajka Oleg Vasilevich

Elikhanov Adlan Majrbekovich

Glazova Natalya Vladimirovna

Dudnik Olga Gennadevna

Melnikova Yana Viktorovna

Dates

2018-06-21—Published

2016-07-01—Filed