Method for obtaining molecular X-chromosome STR markers for identifying an unknown individual and determining biological relationship for working with samples of small amounts of DNA and a set of oligonucleotides for its implementation Russian patent published in 2023 - IPC C12Q1/6869 

FIELD: biotechnology, forensic science and forensics, genetic research.

SUBSTANCE: method for obtaining molecular STR markers of the X chromosome by multiplex amplification to identify an unknown individual and determine biological relationship to work with samples of small amounts of DNA and a set of synthetic oligonucleotide primers for multiplex polymerase chain reaction (PCR). The genotyping kit includes a composition formed by 15 pairs of synthetic original oligonucleotide primers for STR loci of the human X chromosome.

EFFECT: allows you to successfully analyze small amounts of biological material to identify an unknown individual and determine biological relationship in the Russian population with high sensitivity, specificity and accuracy.

5 cl, 2 tbl, 1 ex