FIELD: chemistry.
SUBSTANCE: group of inventions relates to biotechnology and food industry. Disclosed is a method of producing a biocatalyst for re-esterification of fats. The method includes amination of granular silica gel or silicon dioxide with particle size of 0.3-1.0 mm with aminopropyltriethoxysilane. The obtained aminated support is treated with aqueous solution of glutaric aldehyde or glyoxal with concentration of 2.0 wt % or 5.0 wt % for 2 hours. The catalyst is immobilised on the treated catalyst by recycling through said support a solution of thermally stable lipase of Geobacillus lituanicus bacteria in a phosphate buffer at temperature of 0°C or 4°C at pH 6.5 for 12 hours. The obtained biocatalyst is then washed with aqueous solution of tris(hydroxymethyl)aminomethane hydrochloride. Also disclosed is a biocatalyst for re-esterification of fats obtained using said method.
EFFECT: simple method and obtaining a biocatalyst having high catalytic activity and high mechanical strength.
2 cl, 4 ex
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Authors
Dates
2014-09-20—Published
2011-11-11—Filed