BIOCATALYST FOR RE-ESTERIFICATION OF FATS AND METHOD FOR PRODUCTION THEREOF Russian patent published in 2014 - IPC C12N11/14 C12N9/20 C11C3/10 

Abstract RU 2528778 C2

FIELD: chemistry.

SUBSTANCE: group of inventions relates to biotechnology and food industry. Disclosed is a method of producing a biocatalyst for re-esterification of fats. The method includes amination of granular silica gel or silicon dioxide with particle size of 0.3-1.0 mm with aminopropyltriethoxysilane. The obtained aminated support is treated with aqueous solution of glutaric aldehyde or glyoxal with concentration of 2.0 wt % or 5.0 wt % for 2 hours. The catalyst is immobilised on the treated catalyst by recycling through said support a solution of thermally stable lipase of Geobacillus lituanicus bacteria in a phosphate buffer at temperature of 0°C or 4°C at pH 6.5 for 12 hours. The obtained biocatalyst is then washed with aqueous solution of tris(hydroxymethyl)aminomethane hydrochloride. Also disclosed is a biocatalyst for re-esterification of fats obtained using said method.

EFFECT: simple method and obtaining a biocatalyst having high catalytic activity and high mechanical strength.

2 cl, 4 ex

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RU 2 528 778 C2

Authors

Novikov Andrej Aleksandrovich

Kotelev Mikhail Sergeevich

Semenov Anton Pavlovich

Gushchin Pavel Aleksandrovich

Ivanov Evgenij Vladimirovich

Sorokina Ksenija Nikolaevna

Rozanov Aleksej Sergeevich

Vinokurov Vladimir Arnol'Dovich

Dates

2014-09-20Published

2011-11-11Filed