Francisella tularensis 15/23-1ΔrecA STRAIN HAVING REDUCED REACTOGENICITY FOR PRODUCING LIVE TULAREMIA VACCINE AND METHOD FOR PRODUCTION THEREOF Russian patent published in 2015 - IPC C12N1/20 C12N15/01 A61K39/02 

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology and the Francisella tularensis 15/23-1ΔrecA strain, as well as a method of producing same. The described strain is genetically labelled: has only one copy of the iglC gene and a deleted recA gene. The strain is obtained from the Francisella tularensis 15 NIIEG vaccine strain through successive allelic exchange of one of the two copies of the iglC gene then the recA gene on their deleted versions using a suicide vector plasmid, which is inserted into the cell of the strain by transformation followed by collection of F. tularensis strain cells based on a chloramphenicol resistance attribute and further selection of modified strains on a medium with saccharose.

EFFECT: invention enables to obtain a strain with reduced reactogenicity and enables use thereof as a live tularemia vaccine.

2 cl, 6 dwg, 8 tbl, 12 ex