FIELD: pharmaceutics.
SUBSTANCE: invention relates to pharmaceutical industry, specifically to a method of producing recombinant strain Escherichia coli N106 (pM.AgsI). Recombinant bacterial strain Escherichia coli N106 (pM.AgsI) is obtained by transformation of cells of Escherichia coli strain RR1 plasmid pM.AgsI-106, producing DNA-methyltransferase M.AgsI, recognising DNA nucleotide sequence 5′-TTSAA-3′ and methylating external residue of adenine of said sequence to form a 5′-TTSA (m6A)-3′.
EFFECT: method described above enables to obtain recombinant strain Escherichia coli N106 (pM_AgsI) producing highly active DNA methyltransferase of given specificity.
1 cl, 5 dwg, 1 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT BACTERIAL STRAIN ESCHERICHIA COLI N16 (PM.ALUBI) - PRODUCER OF DNA METHYLTRANSFERASE M.ALUBI | 2015 |
|
RU2603086C1 |
| RECOMBINANT BACTERIAL STRAIN Escherichia coli N42 (pElmI) - PRODUCER OF METHYL-DEPENDANT SITE-SPECIFIC ENDONUCLEASE ElmI | 2015 |
|
RU2597987C1 |
| NOVEL VERSIONS OF POLYPEPTIDE PapM OF BACTERIA GENUS Streptomyces | 2003 |
|
RU2351608C2 |
| RECOMBINANT STRAIN OF BACTERIA Escherichia coli N41 (pBpuN4/MR) -PRODUCER OF SITE-SPECIFIC ENDONUCLEASE OF RESTRICTION BpuN4I | 2013 |
|
RU2529362C1 |
| RECOMBINANT PLASMID DNA pEstPc, ENCODING POLYPEPTIDE WITH PROPERTIES OF ESTERASE Psychrobacter cryohalolentis K5, Escherichia coli BACTERIA STRAIN - PRODUCER OF POLYPEPTIDE WITH PROPERTIES OF ESTERASE Psychrobacter cryohalolentis K5 | 2011 |
|
RU2478708C1 |
| AlpA LYTIC PROTEASE OF LYSOBACTER SP XLI BACTERIA, DNA FRAGMENT CODING AlpA LYTIC PROTEASE OF LYSOBACTER SP XLI BACTERIA, AND METHOD OF OBTAINING AlpA LYTIC PROTEASE OF LYSOBACTER SP XLI BACTERIA | 2009 |
|
RU2407782C2 |
| LYTIC PROTEASE AlpB OF BACTERIUM Lysobacter sp XLI, DNA FRAGMENT, CODING LYTIC PROTEASE AlpB OF BACTERIUM Lysobacter sp XLI, AND METHOD FOR PRODUCTION OF LYTIC PROTEASE AlpB OF BACTERIUM Lysobacter sp XLI | 2009 |
|
RU2408725C2 |
| PILL 435 RECOMBINATION PLASMA DEOXYRIBONUCLEIC ACID CODING SYNTHESIS OF T4 BACTERIOPHAGE DNA-LIGASE,METHOD OF CONSTRUCTING AND E.COLIBKM B-1449 STRAIN - PRODUCEROF T4 BACTERIOPHAGE DNA-LIGASE | 0 |
|
SU1122003A1 |
| RECOMBINANT PLASMID DNA pEst877 DETERMINING EXPRESSION OF POLYPEPTIDE WITH ACTIVITY OF ESTERASE Psychrobacter cryohalolentis K5 ON CELL SURFACE Escherichia coli, AND BACTERIA STRAIN Escherichia coli BL21 (DE3) pLysS/pEst877-PRODUCER OF POLYPEPTIDE WITH ACTIVITY OF ESTERASE PSYCHROBACTER CRYOHALOLENTIS K5 ON CELL SURFACE | 2013 |
|
RU2526213C1 |
| RECOMBINANT PLASMID DNA pFK2 PROVIDING SYNTHESIS OF RECOMBINANT PEPTIDE BEING HUMAN KAPPA CASEIN FRAGMENT ANALOGUE, METHOD FOR PREPARING RECOMBINANT PEPTIDE, AND RECOMBINANT PEPTIDE, HUMAN KAPPA CASEIN FRAGMENT ANALOGUE EXHIBITING APOPTOTIC ACTIVITY IN RELATION TO MALIGNANT CELLS | 2009 |
|
RU2401307C1 |
