FIELD: pharmaceutics.
SUBSTANCE: invention relates to pharmaceutical industry, specifically to a method of producing recombinant strain Escherichia coli N106 (pM.AgsI). Recombinant bacterial strain Escherichia coli N106 (pM.AgsI) is obtained by transformation of cells of Escherichia coli strain RR1 plasmid pM.AgsI-106, producing DNA-methyltransferase M.AgsI, recognising DNA nucleotide sequence 5′-TTSAA-3′ and methylating external residue of adenine of said sequence to form a 5′-TTSA (m6A)-3′.
EFFECT: method described above enables to obtain recombinant strain Escherichia coli N106 (pM_AgsI) producing highly active DNA methyltransferase of given specificity.
1 cl, 5 dwg, 1 tbl, 3 ex
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