FIELD: biotechnologies.
SUBSTANCE: expression vector of a target product includes a polynucleotide coding the target product, a polynucleotide coding the first selective marker (sm I), a polynucleotide coding the second selective marker (sm II), which differs from the first selective marker (sm I). Action of the other selective marker has at least partial influence on action of selective marker (sm I) or (sm II). Selective markers (sm I) and (sm II) participate in folate metabolism. The first selective marker (sm I) is a receptor of folate of alpha of a human being (huFolR). The second selective marker (sm II) is dehydrofolate reductase or its functional version or derivative.
EFFECT: invention allows obtaining an effective system of extraction of host cells producing a target product at a high level.
8 cl, 14 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| SYSTEM OF EXPRESSION VECTORS COMPRISING TWO SELECTABLE MARKERS | 2014 |
|
RU2649361C2 |
| METHODS FOR SELECTING EUKARYOTIC CELLS, EXPRESSING HETEROLOGOUS PROTEIN | 2010 |
|
RU2551229C2 |
| ORGANIC COMPOUNDS | 2008 |
|
RU2502802C2 |
| NOVEL SELECTIVE VECTORS AND METHODS FOR SELECTING EUKARYOTIC HOST CELLS | 2014 |
|
RU2716977C2 |
| NOVEL EUKARYOTIC CELLS AND METHODS FOR PREPARING THEREOF FOR RECOMBINANT EXPRESSION OF PRODUCT OF INTEREST | 2014 |
|
RU2712507C2 |
| NOVEL EUKARYOTIC CELLS AND METHODS FOR PREPARING THEM FOR RECOMBINANT EXPRESSION OF A PRODUCT OF INTEREST | 2014 |
|
RU2720525C2 |
| DISPLAY ON POLYPEPTIDE ISOFORM CELL SURFACE ON BASIS OF TERMINATION CODON READTHROUGH | 2009 |
|
RU2528858C2 |
| EXPRESSION VECTOR OF MAMMALS | 2008 |
|
RU2494147C2 |
| OPTIMIZED EXPRESSION CASSETTE FOR HIGH-OUTPUT POLYPEPTIDE EXPRESSION | 2013 |
|
RU2682884C2 |
| PROMOTER | 2008 |
|
RU2491344C2 |
