FIELD: biotechnology.
SUBSTANCE: invention relates to cellular biology and biotechnology, specifically to the production of a mammalian host cell for expression of the desired product and a method for producing the desired product. Method comprises introducing into the host cell of a mammal at least a polynucleotide encoding the desired product, a polynucleotide encoding a first selectable marker that is a folate transporter capable of importing at least one folate into a host cell and a polynucleotide encoding a second selectable marker that is DGFR or its functional derivative. Subsequent cultivation yields the desired product.
EFFECT: invention allows to obtain a host cell that efficiently expresses the desired product.
16 cl, 10 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| EXPRESSION VECTOR SYSTEM CONTAINING TWO SELECTIVE MARKERS | 2010 |
|
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| METHODS FOR SELECTING EUKARYOTIC CELLS, EXPRESSING HETEROLOGOUS PROTEIN | 2010 |
|
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| ORGANIC COMPOUNDS | 2008 |
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| NOVEL SELECTIVE VECTORS AND METHODS FOR SELECTING EUKARYOTIC HOST CELLS | 2014 |
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| NOVEL EUKARYOTIC CELLS AND METHODS FOR PREPARING THEREOF FOR RECOMBINANT EXPRESSION OF PRODUCT OF INTEREST | 2014 |
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| NOVEL EUKARYOTIC CELLS AND METHODS FOR PREPARING THEM FOR RECOMBINANT EXPRESSION OF A PRODUCT OF INTEREST | 2014 |
|
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| DISPLAY ON POLYPEPTIDE ISOFORM CELL SURFACE ON BASIS OF TERMINATION CODON READTHROUGH | 2009 |
|
RU2528858C2 |
| EXPRESSION VECTOR OF MAMMALS | 2008 |
|
RU2494147C2 |
| OPTIMIZED EXPRESSION CASSETTE FOR HIGH-OUTPUT POLYPEPTIDE EXPRESSION | 2013 |
|
RU2682884C2 |
| PROMOTER | 2008 |
|
RU2491344C2 |
