FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to molecular biology and genetic engineering. What is presented is a method for selecting an eukaryotic host cell expressing a desired level of polypeptide of interest involving cell transfection by heterologous nucleic acid containing at least one cartridge containing at least one first polynucleotide coding the polypeptide of interest, a stop codon arranged towards the expression in relation to the first polynucleotide, a second polynucleotide arranged towards the expression in relation to the stop codon coding an immunoglobulin transmembrane anchor domain, culturing host cells for the expression of the polypeptide of interest so that a portion of the disclosed polypeptide is expressed in the form of a fused polypeptide containing the immunoglobulin transmembrane anchor domain; this fused polypeptide is exposed on the surface of the above host cell, selecting the cell by the presence or the amount of the fused polypeptide exposed on the cell surface.
EFFECT: invention can be used in biotechnology for selecting the high-production cell lines.
15 cl, 8 tbl, 11 ex
