FIELD: biotechnology.
SUBSTANCE: present invention relates to cellular technologies, and can be used to express a polypeptide of interest in CHO cells. By combining a specific polynucleotide sequence 5'UTR and the secretory leader sequence hCD33 in an expression cassette for the expression of a polypeptide of interest and further transformation of the obtained host cell genetic construct, a CHO cell is produced with a higher expression level of the polypeptide of interest as compared to the prototype expression cassettes.
EFFECT: invention makes it possible to produce a polypeptide of interest in high yield.
15 cl, 5 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| SYNTHETIC 5'UTR (UNTRANSLATED REGIONS) EXPRESSION VECTORS AND METHOD OF INCREASING TRANSGENIC EXPRESSION | 2008 |
|
RU2524431C2 |
| EXPRESSION METHOD | 2014 |
|
RU2731717C2 |
| SIGNAL PEPTIDES TAT FOR PRODUCTION OF PROTEINS IN PROCARYOTES | 2008 |
|
RU2487937C2 |
| NOVEL SELECTIVE VECTORS AND METHODS FOR SELECTING EUKARYOTIC HOST CELLS | 2014 |
|
RU2716977C2 |
| PLANT REGULATORY ELEMENTS AND THEIR APPLICATION | 2013 |
|
RU2639275C2 |
| HOST CELLS AND WAYS TO USE | 2014 |
|
RU2663587C2 |
| REGULATORY SEQUENCE TO INCREASE GENE EXPRESSION | 2022 |
|
RU2804421C1 |
| DISPLAY ON POLYPEPTIDE ISOFORM CELL SURFACE ON BASIS OF TERMINATION CODON READTHROUGH | 2009 |
|
RU2528858C2 |
| IMPROVED PRODUCTION OF PROTEIN IN BACILLUS | 2008 |
|
RU2515112C2 |
| NOVEL EUKARYOTIC CELLS AND METHODS FOR PREPARING THEREOF FOR RECOMBINANT EXPRESSION OF PRODUCT OF INTEREST | 2014 |
|
RU2712507C2 |
